Primary photoreaction of photoactive yellow protein
نویسندگان
چکیده
منابع مشابه
The primary photoreaction of photoactive yellow protein (PYP): anisotropy changes and excitation wavelength dependence
The absorption and stimulated emission changes in the first 535 ps of the PYP photocycle can be described by four life times of 0.7, 6.3, and 220 ps and long lived. Two intermediates, I0 and I z 0, were identified. We did not obtain indications for a significant excitation wavelength dependent primary photochemistry as found in low temperature absorption spectroscopy. The anisotropy of the prim...
متن کاملRole of protein in the primary step of the photoreaction of yellow protein.
We show the unexpectedly important role of the protein environment in the primary step of the photoreaction of the yellow protein after light illumination. The driving force of the trans-to-cis isomerization reaction was analyzed by a computational method. The force was separated into two different components: the term due to the protein-chromophore interaction and the intrinsic term of the chr...
متن کاملConcerted motions in the photoactive yellow protein.
Molecular dynamics simulations have been performed with the aim of identifying concerted backbone motions in the photoactive yellow protein. Application of the essential dynamics method revealed large, chromophore-linked fluctuations of the protein in the ground state, as well as in a form containing the isomerized chromophore. Various loops become more mobile upon isomerization of the chromoph...
متن کاملPrimary steps of the photoactive yellow protein: isolated chromophore dynamics and protein directed function.
The cycle of the photoactive yellow protein (PYP) has been extensively studied, but the dynamics of the isolated chromophore responsible for transduction is unknown. Here, we present real-time observation of the dynamics of the negatively charged chromophore and detection of intermediates along the path of trans-to-cis isomerization using femtosecond mass selection/electron detachment technique...
متن کاملPrimary photodynamics of a biomimetic model of photoactive yellow protein (PYP).
The present work aims at characterizing the photophysical behavior of a first biomimetic cyclodextrin model (CD-PYP1) of the photoactive site of photoactive yellow protein (PYP). The hydrophobic cyclodextrin cavity in which the chromophore self-includes, mimics its local environment within the protein. The photoinduced behavior of deprotonated CD-PYP1 (dp-CD-PYP1) has been probed by femtosecond...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2001
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.41.s64_1